Three-dimensional cultivation technique of black fungus

The technique of three-dimensional cultivation of black fungus in the big bag not only solves the shortcomings of low yield and easy pollution of black wood ear bag cultivation, but also has the advantages of being free from climatic conditions, sites, resources, funds, etc., saving labor, material saving, high yield and excellent quality. It has low cultivation difficulty, short cycle and high efficiency. The technical points are now introduced as follows.

First, the cultivation site. Can use vegetable greenhouses, idle venues, balconies, roofs, trees and trees under the shade of the trees, close to the water source, well ventilated, away from pollution sources

Second, the cultivation season. The best earing period is when the local temperature is stable at 15-25 °C.

Third, raw materials and preparation.

A. Cottonseed hull 90% bran 5% corn flour 3% lime powder 1% gypsum powder 1%

B. Corn powder 80% bran 10% corn flour 5% soy flour 2% quicklime powder 2% gypsum powder 1%

Fourth, bagging. The bag can be selected from a 22 x 38 x 0.04 cm high density polyethylene cylinder. When loading, it is necessary to tighten tightly, and it is not possible to make a gap between the bag and the material, and the tie should be tight to prevent the formation of a water bag, so that the cultivation fails.

5. Sterilization. Steam sterilization is generally used, and boilers of different specifications are selected according to the amount of production. Household cultivation can be converted into a steam generator using iron drums. During the reforming, a mouth is cut in one side of the iron drum, and a steel pipe having a diameter of 2 cm is inserted into the other side of the barrel from the opening, and the opening is tightly welded to the steel pipe. Open a 4 cm port at a different position on the same side of the opening and use a 4 cm short steel tube (slightly inserted into the barrel wall) to weld tightly to the opening. 2 cm tube is used as the water supply pipe, 4 cm tube is used as the exhaust pipe, and the brick is used as the stove. The barrel is placed on the stove. After the water is added to the barrel, the fire can be burned in the stove to make the steam. Always add water in the middle of sterilization to avoid drying the barrel and affecting the amount of vapor generated.

Six, vaccination. After the vaccination personnel enter the inoculation room, they can first scrub the hands with 75% alcohol cotton balls, then every 3 people, one person excavates the bacteria from the bottle, and the two unpack and bag. Two pieces of red jujube-sized strains can be connected to both ends of each bag. Once all the bacteria bags have been connected, do not arbitrarily enter and exit at the same time, otherwise you need to re-fumigate and sterilize before you can operate again.

Seven, the management of the bacteria. The bacteria bag after inoculation is placed in a clean room or in a greenhouse to protect from light. Keep the temperature at 25-28 °C. Do not ventilate for the first 5 days. The shed can use the method of adding light or shading to increase the temperature. When adding light, the bag should be shaded with black film or straw. After 5 days, it is suitable for proper ventilation and keep the humidity below 70%. After 40-50 days of cultivation, the hyphae can be transferred to the ear management after the bag is full.

Eight, ear management.

A. Site management. If the earing site is selected in the shed, a small soil of 15 cm wide and 15-20 cm high can be made in the shed, and the soil spacing is 80 cm. In the outdoor, the soil can also be made in this way, as in hardening. On the ground, you can lay a layer of bricks at the above distance.

B. The opening of the bacteria bag. Once the site is ready, the ear can be cut out of the bag. First wash the bag with 1% potassium permanganate solution or 0.2% clomazone solution, then use a sharp blade to open 4-6 "V" shaped mouths on both ends. The upper diameter is about 2 cm.

C. Place the bacteria bag. After the mouth is cut, place the bag on the soil. The distance between the bag and the bag is 5-8 cm. Fill the soil with soil. If it is hardened, it can be filled without filling. The filling should be added to the upper part of the bag. 3 cm thick soil layer, and then set the second layer of bacteria bag according to the first layer. If you don't fill the soil, put two small bamboo stalks with a diameter of 2-3 cm on the first layer of bags, and then put a second layer of bacteria bags. In this way, 6-8 layers are placed in sequence. Finally, a layer of mulch is laid near the mound to prevent the soil from splashing onto the ear when spraying.